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Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes BJMBR
Costa,L.O.B.; Mendes,M.C.; Ferriani,R.A.; Moura,M.D.; Reis,R.M.; Silva de Sá,M.F..
The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Steroid in follicular fluid; Maturity of oocytes; In vitro fertilization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100021
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Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas BJMBR
Veiga-Castelli,L.C.; Rosa e Silva,J.C.; Meola,J.; Ferriani,R.A.; Yoshimoto,M.; Santos,S.A.; Squire,J.A.; Martelli,L..
Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endometrioma; Endometriosis; Comparative genomic hybridization; Chromosomal imbalances.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014
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Increased expression of ID2, PRELP and SMOC2 genes in patients with endometriosis BJMBR
Araujo,F.M.; Meola,J.; Rosa-e-Silva,J.C.; Paz,C.C.P.; Ferriani,R.A.; Nogueira,A.A..
Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis in the proliferative phase of menstrual cycle. Analysis was performed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Endometriosis; PRELP; SMOC2; ID2; Real-time PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700605
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Positive correlation of serum leptin with estradiol levels in patients with polycystic ovary syndrome BJMBR
Mendonça,H.C.; Montenegro Junior,R.M.; Foss,M.C.; Silva de Sá,M.F.; Ferriani,R.A..
Patients with polycystic ovary syndrome (PCOS) usually are obese, insulin resistant and hyperinsulinemic. The known association between leptin, obesity andinsulin action suggests that leptin may have a role in PCOS but this has only been addressed peripherally. This study was designed to assess the relationship between serum leptin and the anthropometric, metabolic and endocrine variables of obese (body mass index, BMI ³30 kg/m²) and non-obese (BMI <30 kg/m²) PCOS patients. Twenty-eight PCOS patients and 24 control women subdivided into obese and non-obese groups were evaluated. Leptin, androgens, lipids, gonadotrophins and insulin-glucose response to the oral glucose tolerance test were measured by radioimmunoassay in all participants. The assays were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptin; Polycystic ovary syndrome; Insulin; Insulin sensitivity; Estrogen.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500015
Registros recuperados: 4
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